Phenotypic characterization and complete genome of a tumorigenic pathobiont Escherichia coli LI60C3.

ABSTRACT:

Background

Symbiotic microbes benefit the host, but the emergence of pathobionts leads to disease. An invasive Escherichia coli LI60C3, isolated from mouse colonocytes, shows colitogenic and tumorigenic properties. Despite extensive research on the role of microbiota in colorectal cancer (CRC) development, the genetic markers associated with this pathobiont remain elusive. The objective is to characterize the tumorigenic E. coli through whole-genome sequencing (WGS) and phenotypic assays, and validate their presence in human CRC.

Methods

The intracellular bacterial counts and proliferation rates of human intestinal epithelial cells were evaluated after exposure to various E. coli strains. Tumor burden was assessed in mice orally administered LI60C3. WGS of LI60C3 was perform

SUBMITTER: Yu LC 

PROVIDER: S-EPMC12366417 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature