Phosphorylation-Inducing Molecules for Regulating Dynamic Cellular Processes.

ABSTRACT: Dynamic cellular processes often employ protein phosphorylation for rapid information transfer within and between cells. Phosphorylation-inducing chimeric small molecules (PHICS) have been developed for targeted protein phosphorylation by on-demand inducing a kinase-protein pairing. However, widespread application of PHICS has been limited as previously reported PHICS that recruited AMP-activated protein kinase (AMPK) required serum starvation and target protein overexpression, recruited only a few of the potential AMPK complexes, and exhibited poor dose and temporal control. Herein, we report an AMPK PHICS platform that operates under physiological conditions (i.e., no serum starvation), recruits multiple AMPK complexes, and can induce target protein phosphorylation with dose and temporal

SUBMITTER: Pergu R 

PROVIDER: S-EPMC12366445 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature