Targeted next-generation sequencing of candidate regions identified by GWAS revealed SNPs associated with IBD in GSDs.

ABSTRACT: Canine Inflammatory bowel disease (IBD) is a chronic multifactorial disease, resulting from complex interactions between the intestinal immune system, microbiota and environmental factors in genetically predisposed dogs. Previously, we identified several single nucleotide polymorphisms (SNP) and regions on chromosomes (Chr) 7, 9, 11 and 13 associated with IBD in German shepherd dogs (GSD) using GWAS and FST association analyses. Here, building on our previous results, we performed a targeted next-generation sequencing (NGS) of a two Mb region on Chr 9 and 11 that included 14 of the newly identified candidate genes, to identify potential functional SNPs that could explain these association signals. Furthermore, correlations between genotype and treatment response were estimated. Results rev

SUBMITTER: Peiravan A 

PROVIDER: S-EPMC12366469 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature