Evaluation of a Targeted Drug-Eluting Intravascular Nanotherapy to Prevent Neointimal Hyperplasia in an Atherosclerotic Rat Model.

ABSTRACT: To test the hypothesis that nitric oxide-bearing collagen-targeted nanofibers will target vascular injury and inhibit neointimal hyperplasia in an atherosclerotic rat model. Western blot confirms apolipoprotein E (ApoE) knockout (-/-) status. Serum cholesterol increases three-fold in Sprague Dawley (SD) ApoE-/- vs. wt SD rats (291.7±22.3 vs. 105.0±3.6 mg/dL, p<0.05). Oxidative stress markers are elevated in SD ApoE-/- vs. wt SD strains (p=0.002). Oil Red O staining shows lipid-rich lesions in SD ApoE-/- aortas. Transmission electron microscopy shows co-assembled peptide amphiphiles (PA) form nanofibers. Fluorescence microscopy shows targeting of collagen-binding peptide (CBP)-S-nitrosyl(SNO)-PA nanofiber to arteries 20 minutes after injury, while uninjured carotid and non-targeted SNO-PA n

SUBMITTER: Newton ER 

PROVIDER: S-EPMC12366775 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature