Crotoxin Elicits Differential Increases in Macrophage Lipid Droplet Formation In Vitro Modulated during Infection.

ABSTRACT: Leishmania spp. is an obligate intracellular parasite that primarily infects macrophages. During infection, leukocytes are activated, culminating in the formation of lipid droplets (LDs), which are sites for the synthesis of eicosanoids that modulate the immune response. Crotoxin (CTX), the major toxin derived from (Cdt) snake venom, exhibits pro-inflammatory properties by promoting LD formation and further eicosanoid production. The aim of the present study was to analyze the effects of CTX on the formation of LDs in uninfected and -infected macrophages. Cells were treated with CTX (2.4 and 4.8 μg/mL) and infected or not with parasites, before analyzing LD formation. The kinetic effect of CTX in uninfected macrophages demonstrated a time-dependent and progressive formation of LDs. CTX inh

SUBMITTER: Avila LT 

PROVIDER: S-EPMC12366855 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature