Peripherin-2 and ROM1 Incorporate Directly Into the Rims of Enclosing Photoreceptor Discs Without Accumulating in the Nascent Disc Lamellae.

ABSTRACT:

Purpose

Oligomeric complexes of peripherin-2 and ROM1 support the rim structure of membrane discs stacked inside the light-sensitive outer segment of vertebrate photoreceptor cells. We investigated the route by which peripherin-2 and ROM1 reach their destination within the disc rims. We addressed two possible mechanisms: first, whether these proteins accumulate within the lamellae of newly forming discs prior to their enclosure, after which they incorporate into the rims, or second, whether they incorporate directly into the rims of discs undergoing the process of enclosure.

Methods

Subcellular localization of endogenous and myc-tagged peripherin-2, ROM1, and the cyclic nucleotide-gated (CNG) channel in rod photoreceptors of wild type mice was analyzed by their co-immunostai

SUBMITTER: Klementieva NV 

PROVIDER: S-EPMC12366860 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature