Predicting Mechanosensitive T Cell Expansion from Cell Spreading.

ABSTRACT: Variability in T cell performance presents a major challenge to adoptive cellular immunotherapy (ACT). This includes expansion of a small starting population into therapeutically effective numbers, which can fail due to differences between individuals and disease states. Intriguingly, modulating the mechanical stiffness of materials used to activate T cells can rescue subsequent expansion. However, the magnitude of this effect and the optimal stiffnesses differ between individuals, complicating the use of mechanosensing to improve cell production. The ability to predict this long-term, substrate-dependent expansion from a short-term assay would accelerate the deployment of immunotherapy. Here, it is demonstrated that short-term cell spreading predicts subsequent, mechanosensitive expansion

SUBMITTER: Wang X 

PROVIDER: S-EPMC12367345 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature