Benchmarking 16S rRNA Gene-Based Approaches to Bacterial Taxonomy Assignment Based on Amplicon Sequencing With Illumina and Oxford Nanopore.

ABSTRACT: Research investigating the microbial community of an ecosystem or animal can involve a range of methodologies, including sequencing technology, bioinformatic software and taxonomy database. Researchers may utilise short-read sequencing on Illumina MiSeq or long-read sequencing on platforms like Oxford Nanopore to obtain different research outcomes, for example, enhanced identification of microbes at species or strain level with Nanopore. However, replicability across these techniques is not well studied, while the technique used to process reads into microbial taxa may also result in different taxonomy assignments. In this study, we analyse an existing, real-world dataset which had low genus-level identification with Illumina sequencing and analysis with the SILVA database and compare sequ

SUBMITTER: Hoffbeck C 

PROVIDER: S-EPMC12367389 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature