Ca²⁺-pumping by PMCA-neuroplastin complexes operates in the kiloHertz-range.

ABSTRACT: Ca²⁺-ATPases in the plasma membrane extrude Ca²⁺ ions from the cytosol to the extracellular space thereby terminating Ca²⁺-signals and controlling Ca²⁺-homeostasis in any type of cell. Recently, these Ca²⁺-pumps have been identified as protein complexes of the transporting subunits PMCAs1-4 and the single-span membrane proteins Neuroplastin (NPTN) or Basigin that are obligatory for efficient trafficking of the pump complexes to the surface membrane. Quantitative investigation of the pumping velocity controlling the time course of Ca²⁺-signals, however, has remained unresolved. Here we show, using Ca²⁺-activated K⁺ channels as fast native reporters of intracellular Ca²⁺ concentration(s) togeth

SUBMITTER: Constantin CE 

PROVIDER: S-EPMC12368035 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature