Intracristal space proteome mapping using super-resolution proximity labeling with isotope-coded probes.

ABSTRACT: Proximity labeling with engineered ascorbate peroxidase (APEX) has been widely used to identify proteomes within various membrane-enclosed subcellular organelles. However, constructing protein distribution maps between two non-partitioned proximal spaces remains challenging with the current proximity labeling tools. Here, we introduce a proximity labeling approach using isotope-coded phenol probes for APEX labeling (ICAX) that enables the quantitative analysis of the spatial proteome at nanometer resolution between two distinctly localized APEX enzymes. Using this technique, we identify the spatial proteomic architecture of the mitochondrial intracristal space (ICS), which is not physically separated from the peripheral space. ICAX analysis further reveals unexpected dynamics of the mitoch

SUBMITTER: Kang MG 

PROVIDER: S-EPMC12368266 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature