Molecular Insights into the Interaction between CD147 and the SARS-CoV‑2 Spike Protein.

ABSTRACT: Cluster of differentiation 147 (CD147), a transmembrane glycoprotein, has been identified as a potential auxiliary receptor for the SARS-CoV-2 spike protein (SP), contributing to COVID-19 infection. However, the detailed binding characteristics of this interaction remain unclear. Here, we characterized SP-CD147 binding using Enzyme-Linked Immunosorbent Assay (ELISA) and single-molecule force spectroscopy (SMFS) under varying contact times and temperatures. While SP binds CD147 with lower force than angiotensin-converting enzyme 2 (ACE2) at normal body temperatures, its binding strength to CD147 significantly increases under fever-like conditions, contrasting with SP-ACE2 interactions, which weaken at elevated temperatures. We further investigated the role of heparin in modulating this inte

SUBMITTER: Gonzalez-Serrano MS 

PROVIDER: S-EPMC12368639 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature