Characterization of Fatty Acid Photodecarboxylase in Zeolitic Imidazolate Frameworks.

ABSTRACT: Fatty acid photodecarboxylase (FAP) was recently discovered in microalgae and is a photoenzyme that harvests sunlight to convert long-chain fatty acids to hydrocarbons. The hydrophobicity of the substrates and photoproducts strongly affects the stability of FAP in vitro studies. Here, we incorporated zeolitic imidazole frameworks (ZIFs) as structural protecting cages and characterized FAP (cvFAP) in ZIFs. Due to the preparation conditions and the surface properties, ZIFs with a hydrophobic surface (ZIF-8) form a cluster-like packed morphology, and ZIFs with a hydrophilic surface (ZIF-90) form a hexagon-like morphology. However, both ZIFs compress FAP and lead to a more hydrophobic active-site environment and fewer active-site water molecules. The ZIF-90 cages enhance the c

SUBMITTER: Su MS 

PROVIDER: S-EPMC12368644 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature