Isolation, Characterization, and Anticancer Evaluation of Alkaloids from Eumachia montana (Rubiaceae).

ABSTRACT: The dimeric (-)-calycanthine (1), the hexameric (+)-oleoidine (2), the heptameric (+)-caledonine (3), the octameric (+)-eumatanine (4), and a tricyclic pyrroloquinoline alkaloid, eumatricine (5), are alkaloids isolated from the leaves of ; compounds 4 and 5 are novel, previously uncharacterized alkaloids. Preliminary anticancer assays, including MTT, cell count, and clonogenic assays, revealed potent inhibition of cell growth and colony formation by compounds 2-4 against seven carcinoma cell lines (including breast, colorectal, lung, and glioblastoma multiforme cell lines). The GI50 values of compounds 2-4 ranged from 0.1 to 2.7 μM, and potent cytotoxic activity against HCT-116 and MCF-7 was observed (3 > 4 > 2). Cell cycle analysis and apoptosis assays revealed that compound 4 evokes prof

SUBMITTER: Shuai Y 

PROVIDER: S-EPMC12368673 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature