The Role of IgE in Crohn's Disease by Impairing the Capacity of Plasmacytoid Dendritic Cells to Generate FOXP3⁺ Tregs.

ABSTRACT:

Background

A causal relationship between Crohn's disease (CD) and asthma is reported, but the underlying mechanisms are not fully understood. We sought to investigate the role of IgE and IgE-mediated pathways in the pathophysiology of CD.

Methods

20 CD patients, 10 allergic patients without inflammatory bowel disease, and 10 healthy donors (HD) were included in the study. Total serum IgE was quantified by ELISA. Circulating IgE⁺ and FcεRIα⁺ immune cells, as well as specific CD4⁺ T cell populations, were determined by flow cytometry. Gene set enrichment signatures from available single-cell (sc)RNAseq datasets of the intestine from CD patients were analyzed. Purified plasmacytoid dendritic cells (pDCs) from CD patients were cocultured with naï

SUBMITTER: de la Rocha-Munoz A 

PROVIDER: S-EPMC12368764 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature