Direct single cell-type gene expression analysis in peripheral blood: novel ratio-based gene expression biomarkers using 2 novel monocyte reference genes (PSAP and CTSS) for detection of bacterial infection.

ABSTRACT:

Background

To determine single-cell-type gene expression in peripheral blood (PB) requires either prior cell sorting or single-cell RNA sequencing. We developed a novel ratio-based biomarker (RBB) called Direct Leukocyte Subpopulation-Transcript Abundance (DIRECT LS-TA) that allows quantification of monocyte-specific gene expression directly from PB without cell sorting.

Methods

DIRECT LS-TA leverages proportional cell counts and differential gene expression profiles among leukocyte subpopulations to identify monocyte-informative genes. Using a new ICEBERG plot (Figure 1) based on a mathematical model of cell-mixture gene expression, we shortlisted genes with 2.5-fold higher expression in isolated monocytes compared to PB, indicating > 50% of transcript contribution by monoc

SUBMITTER: Tang NLS 

PROVIDER: S-EPMC12368773 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature