Three-Dimensional Magnetic Bioprinting Spheroids as an In Vitro Model to Study the Oviductal Physiology.

ABSTRACT: In vitro models to study the oviduct are challenged by cellular dedifferentiation, a complex coculture system for embryo production, limited cell lifespan, and/or very complex methodologies. Hence, we aimed to develop an in vitro oviductal model using the magnetic bioprinting system, a three-dimensional (3D) culture system. Using the bovine epithelial and stromal oviductal cells (BOEC and BOSC, respectively), we produced the Oviductal Magnetic Spheroid (OMS), a duo somatic cell spheroid aggregate with self-organization capacity. The OMS showed to be viable for 21 days and recapitulated the oviductal tissue features after 7 days in culture, such as a simple epithelial cell layer facing outwards, expressing ciliation (acetylated tubulin positive) and secretory marker (oviduct-specific glycop

SUBMITTER: Fontes PK 

PROVIDER: S-EPMC12368830 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature