Passive Membrane Transport Analysis of Drug Mixtures.

ABSTRACT: Membrane transport is fundamental to biological cells and is a major hurdle in the rational design of pharmaceuticals. To measure membrane transport in vitro, most methods focus on simple diffusion of a single analyte across nonbiomimetic interfaces. Membrane engineering has facilitated novel strategies for the reconstitution, characterization, and application of biomimetic membranes. Herein, we define drug mixture analysis, an in vitro, label-free HPLC-MS, droplet interface bilayer (DIB) method, to assess membrane transport of drug mixtures and delineate simultaneous transport mechanisms. We use our method to classify the permeability of drugs from a structurally diverse FDA-approved library. This deep analysis uncovered correlation between determined permeability classifiers and drug pro

SUBMITTER: Strutt R 

PROVIDER: S-EPMC12368836 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature