Subcellular-Resolution Molecular Pathology by Laser Ablation-Rapid Evaporative Ionization Mass Spectrometry.

ABSTRACT: This work demonstrates the combination of ambient laser ablation (LA) with in-source surface-induced declustering, originally developed for rapid evaporative ionization mass spectrometry (REIMS). This combination, termed laser ablation REIMS (LA-REIMS), provides sensitivity, spatial resolution, and chemical coverage comparable to matrix-assisted laser desoprtion ionization (MALDI) but without the requirement for matrix deposition. The atmospheric pressure interface setup was subjected to detailed characterization with regard to geometric and thermal parameters augmented by in-silico flow modeling. The resulting platform was tested using aerosol formed by the infrared laser ablation of tissues. Three different laser systems were successfully employed for ambient mass spectrometric imaging:

SUBMITTER: Simon D 

PROVIDER: S-EPMC12368837 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature