Concentration-Based Analysis of Metal-Induced Tau Fibrillar versus non-fibrillar Aggregation: Implications for Neurotoxicity in Alzheimer's Disease.

ABSTRACT: Tau protein aggregation is the most significant factor in Alzheimer's disease (AD) pathogenesis, and the accumulation of metal ions in the brain is considered a key factor in the development of this disease. Tau protein exhibits two distinct aggregate structures: fibrillar and non-fibrillar aggregates. In this study, we conducted the first detailed study of the interactions of tau protein with three different concentrations of Zn²⁺, Cu²⁺, and Fe³⁺ions. Our findings demonstrate that low concentrations (0.01 mM) of these metal ions promote tau fibrillation, while higher concentrations (1 mM) induce non-fibrillar aggregates. We have investigated the structural changes of tau by using advanced techniques such as SDS-PAGE, DTNB, AFM, CD and fluorescence spectros

SUBMITTER: Irandoust M 

PROVIDER: S-EPMC12368877 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature