Imatinib decreases germ cell survival and germline stem cell proliferation in rodent testis ex vivo and in vitro.

ABSTRACT:

Background

Imatinib and dasatinib are tyrosine kinase inhibitors (TKIs) increasingly used to treat several diseases in both children and adults at fertile age. We have previously shown that imatinib has adverse effects on developing testis, and imatinib-treated male patients have been reported to have reduced sperm counts. However, the cellular level effects of imatinib and dasatinib on adult male germ cells and germline stem cells (mGSCs) have not been thoroughly investigated.

Objectives

To analyze whether imatinib or dasatinib exposure ex vivo and in vitro is harmful to adult male rodent germ cells and mGSCs.

Materials and methods

Seminiferous tubule segments of adult male mouse or rat were cultured in the presence or the absence of imatinib or dasatinib. Stage-spec

SUBMITTER: Eggert A 

PROVIDER: S-EPMC12368934 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature