Development of an Aptamer-Based qPCR Method for the Selective and Rapid Picomolar-Level Detection of Perfluorooctanesulfonic Acid in Water.

ABSTRACT: Per- and polyfluoroalkyl substances (PFAS) are widely recognized as emerging contaminants because they are ubiquitous in various environmental media. Their potential for chronic toxicity after prolonged human exposure is a growing concern. Consequently, there is an urgent need to develop an appropriate technology to efficiently treat and rapidly and consistently monitor PFAS levels. This study reports the development of the first aptamers that can bind to perfluorooctanesulfonic acid (PFOS), with a dissociation constant (K_D) of 6.76 μM, and exhibit a high specificity for PFOS even in the presence of other PFAS. The binding site and mechanism of the prepared aptamers are explored using truncation and molecular dynamics simulations, which show that the lengths of fluorocarb

SUBMITTER: Park J 

PROVIDER: S-EPMC12368987 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature