Drug-Induced Reversible Lysosomal Changes Tracked in Live Cells by Holo-Tomographic Flow Cytometry.

ABSTRACT: Lysosomal storage diseases (LSDs) are genetic disorders caused by enzyme deficiencies that lead to lysosomal dysfunction and progressive cell damage. Accurate visualization and quantification of lysosomal morphology and subcellular localization are essential parameters for understanding the pathology and disease progression of different LSDs, as well as for developing effective therapies. Here, we successfully identified and characterized lysosomes using a holo-tomographic flow cytometry (HTFC) technique, which allows for label-free, high-content, and high-throughput 3D imaging of lysosomal compartments in single live cells. This study could complement traditional gold-standard methods to overcome the actual limitations. Leveraging this technology, we propose quantitative biomarkers of lys

SUBMITTER: Pirone D 

PROVIDER: S-EPMC12369006 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature