Constraint-induced movement therapy combined with mesenchymal stem cell transplantation promotes myelination and functional recovery by inhibiting PRKCD/MEK/ERK pathway in hemiplegic cerebral palsy rats.

ABSTRACT: The core problem of hemiplegic cerebral palsy (HCP) is upper limb motor deficits with high rates of disability. Given the shared goals of stem cell therapy and rehabilitation, this study investigated the synergistic effects of constraint-induced movement therapy (CIMT) and human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) transplantation in promoting motor recovery and elucidates the underlying mechanisms in HCP. The rats were allocated to a control group and HCP groups receiving different interventions (CIMT, hUC-MSCs or combination treatment), namely, the Control, HCP, HCP + CIMT, HCP + MSC, and HCP + CIMT + MSC groups. Motor function was evaluated using rotarod duration, grip strength, and forelimb suspension time. Golgi-Cox staining, transmission electron microscopy, immun

SUBMITTER: Guo X 

PROVIDER: S-EPMC12369169 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature