DNA replication initiation timing is important for maintaining genome integrity.

ABSTRACT: DNA replication is regulated by factors that promote or inhibit initiation. In Bacillus subtilis, YabA is a negative regulator of replication initiation, while the newly identified kinase CcrZ is a positive regulator. The consequences of under-initiation or over-initiation of replication on genome stability remain unclear. In this work, we measure the origin-to-terminus ratio as a proxy for replication initiation activity. We show that ΔccrZ and several ccrZ alleles under-initiate DNA replication, while ablation of yabA or overproduction of CcrZ leads to over-initiation. We find that cells under-initiating DNA replication have few incidents of replication fork stress as determined by the low frequency formation of RecA-GFP foci compared with wild type. In contra

SUBMITTER: Reed TT 

PROVIDER: S-EPMC12369353 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature