Molecular phenotyping for analyzing subtle genetic effects in mice: application to an angiotensinogen gene titration.

ABSTRACT: The angiotensinogen M235T polymorphism in humans is linked to differential expression of the human angiotensinogen gene (AGT) gene and hypertension, but the homeostatic responses resulting from this polymorphism are not known. We therefore investigated how mice respond to five genetically determined levels of mouse angiotensinogen gene (Agt) expression covering the range associated with the M235T variants. By using high-throughput molecular phenotyping, tissue RNAs were assayed for expression of 10 genes important in hypertension. Significant positive and negative responses occurred in both sexes as Agt expression increased twofold, including a three-fold increase in aldosterone synthase expression in adrenal gland, and a two-fold decrease in renin expression in kidney. In males, cardiac e

SUBMITTER: Kim HS 

PROVIDER: S-EPMC123694 | biostudies-literature | 2002 Apr

REPOSITORIES: biostudies-literature