MpmiR319 promotes gemma/gemma cup formation in the liverwort Marchantia polymorpha.

ABSTRACT: miRNAs were initially detected as ~20-22 nt sequences in plants. For decades miRNA-mediated regulation of target gene expression has been characterized in many cases. The sequences of miR159/319 family miRNAs are conserved among land plants. The roles of miR159/319 have been comprehensively characterized in development of dicot and monocot plants. However, their biological function in bryophytes remains enigmatic. A model bryophyte Marchantia polymorpha also encodes miR319 at two loci and expresses the miRNA. We used a CRISPR/Cas9 system to edit genome sequences at MpMIR319a and/or MpMIR319b loci. The mutant lines developed relatively few gemma cups and gemmae, suggesting that MpmiR319 targets MpRKD or MpR2R3-MYB21 transcripts and suppresses their expression. We constructed miR319-resistan

SUBMITTER: Futagami K 

PROVIDER: S-EPMC12369473 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature