Vacuolar phosphatases are essential for efficient nucleotide salvage in Arabidopsis.

ABSTRACT: The salvage pathway that recycles nucleotides from RNA is an important contributor to cellular homeostasis. In Arabidopsis, RNA salvage occurs in the vacuole, in a process started by RNS2. Defects in this pathway lead to constitutive autophagy. How nucleosides are generated from RNS2-catalyzed RNA degradation remains unclear. Using a combination of biochemistry and molecular genetics, we showed that RNS2 produces 2',3'-cAMP and 3'-AMP from poly(A) degradation but only 2',3'-cUMP from poly(U). Mutants lacking PAP26, the major vacuolar acid phosphatase (APase), displayed increased basal autophagy that was rescued by inosine treatment, mirroring rns2 phenotypes. PAP26-deficient vacuoles have lower total APase activity than wild type, but nucleotide processing is not fully disrupted. Further a

SUBMITTER: Liu AY 

PROVIDER: S-EPMC12369478 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature