Functional characterization of antennae-enriched chemosensory protein 4 in emerald ash borer, <i>Agrilus planipennis</i>.

ABSTRACT: Agrilus planipennis is an invasive species that inflicts substantial harm on ash trees (Fraxinus spp.) globally. Elucidating its olfactory mechanisms is essential for devising effective pest management approaches. In this research, we identified chemosensory protein 4 (AplaCSP4) in A. planipennis, which is highly expressed in the antennae of both male and female individuals. Notably, the mRNA expression level of AplaCSP4 in females is 1.9 times higher than that in males. Fluorescence competition binding assays revealed that recombinant AplaCSP4 has a broad binding spectrum, capable of interacting with 11 compounds from various chemical classes such as esters, alkanes, terpenes, terpenoids, and terpenols. The dissociation constants (K _D) for the

SUBMITTER: Li R 

PROVIDER: S-EPMC12369603 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature