The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.

ABSTRACT: We have determined the complete 1,694,969-nt sequence of the GC-rich genome of Methanopyrus kandleri by using a whole direct genome sequencing approach. This approach is based on unlinking of genomic DNA with the ThermoFidelase version of M. kandleri topoisomerase V and cycle sequencing directed by 2'-modified oligonucleotides (Fimers). Sequencing redundancy (3.3x) was sufficient to assemble the genome with less than one error per 40 kb. Using a combination of sequence database searches and coding potential prediction, 1,692 protein-coding genes and 39 genes for structural RNAs were identified. M. kandleri proteins show an unusually high content of negatively charged amino acids, which might be an adaptation to the high intracellular salinity. Previous phylogenetic analysis of 16S RNA sugg

SUBMITTER: Slesarev AI 

PROVIDER: S-EPMC123701 | biostudies-literature | 2002 Apr

REPOSITORIES: biostudies-literature