Sensitivity and Specificity of an All-in-one Cartridge-based Dengue Real-time Reverse Transcription-PCR for Point-of-care Detection and Serotyping of Dengue Virus in Samples from Indonesian Patients.

ABSTRACT:

Background

: Dengue is a systemic, viral, mosquito-borne infection that continues to be a major public health issue in endemic regions in tropical and subtropical climates. Accurate tests for rapid diagnosis in point-of-care settings are important to reduce the fatality rates of severe dengue. We evaluated the diagnostic accuracy of the Standard M10 DENV 1-4 system (SD Biosensor, Gyeonggi, Korea), which is a cartridge-based, automated system that integrates nucleic acid extraction, reverse transcription-PCR (RT-PCR) amplification, and detection of dengue virus (DENV) serotypes.

Methods

: This was a retrospective diagnostic evaluation study. The index test, Standard M10 DENV 1-4, was evaluated using 320 dengue-positive and 279 dengue-negative archived samples. The reference t

SUBMITTER: Santoso MS 

PROVIDER: S-EPMC12370802 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature