Complement Activation and Hemolysis in Non-human Primates Following Transfusion of Genetically Modified Pig Red Blood Cells.

ABSTRACT:

Background

: Pig red blood cells (RBCs) are rapidly eliminated when transfused into nonhuman primates (NHPs) because of immune reactions involving antibody binding and complement activation. We assessed the relationship between post-transfusion hemolysis and complement activation.

Methods

: RBCs for transfusion were prepared from wild-type (WT) and genetically modified pigs and NHPs. After the withdrawal of 25% of the blood volume, NHPs received transfusions of WT (N=4), triple knockout (TKO, N=8), and TKO pig RBCs expressing human CD55 and CD39 (TKO/hCD55.hCD39, N=4). Additional groups received repeated xenotransfusions (ReXTf, N=3), NHP RBC transfusions (N=3), or a saline infusion (N=4). Blood samples were collected at multiple time points to measure Hb and c

SUBMITTER: Kang HJ 

PROVIDER: S-EPMC12370810 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature