Identification of Histoplasma capsulatum transcripts induced in response to reactive nitrogen species.

ABSTRACT: The pathogenic fungus Histoplasma capsulatum escapes innate immune defenses and colonizes host macrophages during infection. After the onset of adaptive immunity, the production of the antimicrobial effector nitric oxide (*NO) restricts H. capsulatum replication. However, H. capsulatum can establish persistent infections, indicating that it survives in the host despite exposure to reactive nitrogen species (RNS). To understand how H. capsulatum responds to RNS, we determined the transcriptional profile of H. capsulatum to *NO-generating compounds using a shotgun genomic microarray. We identified 695 microarray clones that were induced > or = 4-fold upon nitrosative stress. Because our microarray clones were generated from random fragments of genomic DNA, they did not necessarily correspond

SUBMITTER: Nittler MP 

PROVIDER: S-EPMC1237084 | biostudies-literature | 2005 Oct

REPOSITORIES: biostudies-literature