Design of a multi-Epitope mRNA vaccine against Brucella type IV secretion system using reverse vaccinology and immunogenicity approaches.

ABSTRACT: Brucellosis is a zoonotic disease caused by bacteria of the Brucella genus. This study investigates the development of a multi-epitope mRNA vaccine aimed at combating Brucella infections. The approach involves selecting the architecture of the pathogenic type IV secretion system (T4SS) of Brucella using bioinformatics tools and reverse vaccinology methodologies. The candidate proteins chosen for this vaccine include VirB2, VirB3, and VirB6, along with the effector protein BtpA. Key findings include robust antigenicity scores for the candidate proteins (VirB2:0.5685; VirB3:0.5329;VirB6:0.5054;BtpA:0.5575).A variety of tools were essential for identifying potential epitopes suitable for incorporation into the mRNA vaccine, as well as for utilizing appropriate linkers for docking. Molecular d

SUBMITTER: Luo JR 

PROVIDER: S-EPMC12370932 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature