Semiautomatic quantification of 3D Histone H3 phosphorylation signals during cell division in Arabidopsis root meristems.

ABSTRACT: Posttranslational modification of histones during the cell cycle is a major process controlling many aspects of cell division. Among the variety of histone modifications, mitotic phosphorylation of histone H3 at serine 10 (H3S10ph) plays a crucial role, particularly in proper chromosome segregation. Here we aimed at precisely quantifying this phosphorylation dynamics during mitosis in plant cells in order to reveal molecular pathways involved in this process. We describe an analysis pipeline based on 3D image analysis that allows to semiautomatically quantify H3S10ph in mitotic Arabidopsis root cells. We also developed a new method for the compensation of signal attenuation in Z, based on measurement of objects of interest themselves. We show that this new attenuation correction method all

SUBMITTER: Kelemen A 

PROVIDER: S-EPMC12371163 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature