Development of Thymic Organoids Heterotopically to Educate and Induce T Lymphocytes.

ABSTRACT:

Background

Vascularized composite allotransplantation (VCA) is a potential treatment for extensive injuries that replaces defects like-with-like, however allografts are immune-rejectable.

Methods

This study developed in vitro thymic organoids and examined whether donor-derived HSCs could be educated in vivo into T lymphocytes via central tolerance. TECs, TMCs, and HSCs from C57BL/7 (CD45.2⁺) or SJL/L (CD45.1⁺) mice were labeled with cell surface markers and examined by flow cytometry. Co-culturing three cell lines in vitro created thymic aggregates. Aggregates transplanted to C57BL/7 (CD45.2⁺) mice's inguinal regions developed thymic organoids. Immunorejection genes were identified bioinformatically. Western blot, immunofluorescence, and flow

SUBMITTER: Wang X 

PROVIDER: S-EPMC12371559 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature