Effects of Glycerol and Phenolics on <i>Myceliophthora heterothallica</i> Endoxylanase Expressed in <i>K. phaffii</i>.

ABSTRACT: Industrial applications of xylanases in high-temperature settings are limited by enzyme instability. This study evaluated glycerol and phenolic compounds as modulators of the catalytic and structural properties of a recombinant Myceliophthora heterothallica endoxylanase (rMhXyn) expressed in Komagataella phaffii. Glycerol (20% v/v) significantly improved thermostability (5-fold increase in half-life at 55 °C), decreased the activation energy for catalysis, and enhanced structural rigidity as evidenced by molecular dynamics simulations (reduced RMSD and Rg). In contrast, phenolic acids provided only short-term stabilization at moderate temperatures and did not confer structural benefits. Enzyme kinetics revealed that glycerol enhanced catalytic turnover (↑V

SUBMITTER: Zanoni JA 

PROVIDER: S-EPMC12372066 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature