Universal amplification and sequencing of foot-and-mouth disease virus complete genomes using nanopore technology.

ABSTRACT: Foot-and-mouth disease virus (FMDV) is capable of causing explosive outbreaks among domestic and wild cloven-hoofed animals. Genomic characterisation of FMDV is a crucial component of disease control enabling accurate tracing of disease outbreaks to be undertaken. Nanopore sequencing is an affordable and accessible form of high-throughput sequencing (HTS) technology. However, most published methods for FMDV only sequence genomic fragments or focus upon specific lineages. In this study, a universal FMDV sequencing protocol was developed alongside a bespoke analytical pipeline to sequence any FMDV genome in the absence of prior knowledge regarding the identity of the serotype or lineage. Universal multiplex RT-PCRs were used to amplify overlapping tiles encompassing the entire FMDV genome. T

SUBMITTER: Shaw AE 

PROVIDER: S-EPMC12372193 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature