Exposure-inducible genes may contribute to missingness in RNAseq-based gene expression analyses.

ABSTRACT: Missing gene expression values are a common issue in RNAseq-based analyses of gene expression. However, an analysis of genetic and environmental factors contributing to data missingness in RNAseq-based assessment of gene expression has never been conducted. In this study we tried to identify factors in RNAseq data missingness. We used RNAseq data from 66 lung adenocarcinoma tumors and corresponding adjacent normal lung tissues. We found a strong negative association between the gene expression level and missingness, supporting the idea that the borderline expression level is a key contributor to missingness. In a more detailed analysis, the relationship between gene expression and missingness was more complex: while the expected negative association between missingness and the expression l

SUBMITTER: Gorlova OY 

PROVIDER: S-EPMC12373826 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature