Simultaneous online monitoring of viscosity and oxygen transfer rate in shake flask cultures.

ABSTRACT: Shake flasks are among the most relevant culture vessels for early-stage process development of viscous microbial cultures. While online process monitoring systems are available for temperature, pH, biomass concentration, dissolved oxygen tension and respiration activity, online measuring techniques for viscosity are not yet commercially available. Especially during the production of biopolymers and the cultivation of filamentous fungi or bacteria, quantification of fermentation broth viscosity is essential to ensure adequate mixing as well as gas/liquid mass and heat transfer. In this work, a previously developed quantitative online viscosity measurement technique, termed ViMOS, is refined to monitor the apparent viscosity of up to eight shake flask cultures in parallel. In addition, the

SUBMITTER: Hanke R 

PROVIDER: S-EPMC12374283 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature