A platform for multisite immune profiling of premetastatic pancreatic cancer at single-cell resolution.

ABSTRACT:

Background

Pancreatic ductal adenocarcinoma (PDAC) is characterized by exceedingly high rates of metastatic progression, with the liver representing the most common site of distant spread. Here, we established a platform for multisite immune profiling of human PDAC encompassing the tumor, peripheral circulation, and premetastatic liver, to more comprehensively study how various immune subsets might contribute to patient outcomes.

Methods

Tumor, liver, and blood samples were obtained from patients undergoing resection for non-metastatic PDAC. Derived immune cells underwent paired single-cell RNA and TCR sequencing. Immune composition, cell-type functional profiles, and T cell clonal expansion patterns were evaluated across tissue sites.

Results

In total, 106,539 immune

SUBMITTER: Kanu EN 

PROVIDER: S-EPMC12374926 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature