4D structural biology-quantitative dynamics in the eukaryotic RNA exosome complex.

ABSTRACT: Molecular machines play pivotal roles in all biological processes. Most structural methods, however, are unable to directly probe molecular motions. Here, we demonstrate that dedicated NMR experiments can provide quantitative insights into functionally important dynamic regions in very large asymmetric protein complexes. We establish this for the 410 kDa eukaryotic RNA exosome complex that contains ten distinct protein chains. Methyl-group and fluorine NMR experiments reveal site-specific interactions among subunits and with an RNA substrate. Furthermore, we extract quantitative insights into conformational changes within the complex in response to substrate and subunit binding for regions that are invisible in static cryo-EM and crystal structures. In particular, we identify a flexible pl

SUBMITTER: Liebau J 

PROVIDER: S-EPMC12375074 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature