Thymoquinone mediated hedgehog pathway activation and gut microbiota modulation in mouse colitis.

ABSTRACT: To investigate the treatment of ulcerative colitis by thymoquinone (TQ) through the Hedgehog signaling pathway and the composition of intestinal microbiota. A total of 24 male Balb/C mice were randomly divided into blank group, model group, thymoquinone low-dose group (20 mg/kg) and thymoquinone high-dose group (80 mg/kg), with 6 mice in each group. The concentrations of IL-1β, TNF-α, IL-10 and TGF-β in the supernatant of mouse serum were determined by enzyme-linked immunosorbent assay, and the expressions of Shh, Smo, Gli1, Ptch1, Atresin -1 (ZO-1) and Occludin in the mouse colon were detected by RT-qPCR, Western blot and immunohistochemistry. RNA-seq was used to detect changes in the intestinal microbial community of the four groups at the same time. TQ slowed down body weight, reduced d

SUBMITTER: Huang X 

PROVIDER: S-EPMC12375083 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature