Detergent Choice Shapes the Solution Structures of Photosystems I and II: Implications for Crystallization and High-Resolution Studies.

ABSTRACT: Photosystems I (PSI) and II (PSII) are pigment-protein complexes that perform the light-driven charge separation necessary to convert solar energy into a biochemically usable form in a fundamental process called photosynthesis. Small-angle X-ray scattering provides unique structural insights into PSI and PSII in solution under near-physiological conditions. Here, we study the solubilization of PSI and PSII with different detergents, the octaethylene glycol monododecyl ether (C₁₂E₈) and the most commonly used n-dodecyl-β-D-maltoside (DDM). It is noteworthy that the volume of the C₁₂E₈ detergent belt is more compact for PSI and PSII than for DDM. Furthermore, circular dichroism measurements were used to detect thermal destabilization in protein sol

SUBMITTER: Golub M 

PROVIDER: S-EPMC12376099 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature