Comprehensive Characterization of Bihormonal Cells and Endocrine Cell Lineages in Mammalian Pancreatic Islets.

ABSTRACT: Understanding the role and prevalence of bihormonal cells in pancreatic islets and their potential in β-cell restoration is critical but remains ambiguous. Using genetically engineered mouse strains with specific fluorescent markers and advanced imaging flow cytometry, it is found that bihormonal cells are exceedingly rare. Single-cell RNA sequencing reveals that Gcg⁺Ppy⁺ and Gcg⁺Ins⁺ bihormonal cells closely resemble α-cells or PP-cells and α-cells, respectively, indicating they are neither unique lineages nor transitional states. Dual-recombinase lineage tracing further demonstrates that embryonic Gcg⁺Ins⁺ cells resolve into monohormonal α-cells. Applying these insights, the scarcity of bihormonal cells in diabetic mouse m

SUBMITTER: Yu XX 

PROVIDER: S-EPMC12376548 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature