Multivariate Screening and Automated Clustering of Macrophage Immunoreactome to Nanoparticles and Photothermal Therapy.

ABSTRACT: Immunotherapy aims to control the immune system against diseases such as cancer or infections. Nanotechnology is part of the armamentarium to reprogram the immune system in a spatially and temporally controlled manner. However, predicting immune responses using high-throughput tests is challenging due to the immunoreactome's complexity and plasticity. This work presents a fast, machine learning-assisted predictive assay to classify the multifactorial immune responses to any kind of treatments. Engineered human THP-1 monocytes differentiated and polarized into M0, M1, and M2 macrophages are used to monitor nuclear factor Kappa B (NF-kB) and interferon regulatory factor (IRF) pathway activations and gene expression profile in response to metallic nanoparticles (NPs), activated or not by ligh

SUBMITTER: Becharef S 

PROVIDER: S-EPMC12376647 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature