Two-dimensional electrophoresis study of Lactobacillus delbrueckii subsp. bulgaricus thermotolerance.

ABSTRACT: The response of Lactobacillus delbrueckii subsp. bulgaricus cells to heat stress was studied by use of a chemically defined medium. Two-dimensional electrophoresis (2-DE) analysis was used to correlate the kinetics of heat shock protein (HSP) induction with cell recovery from heat injury. We demonstrated that enhanced viability, observed after 10 min at 65 degrees C, resulted from the overexpression of HSP and from mechanisms not linked to protein synthesis. In order to analyze the thermoadaptation mechanisms involved, thermoresistant variants were selected. These variants showed enhanced constitutive tolerance toward heat shock. However, contrary to the wild-type strain, these variants were poorly protected after osmotic or heat pretreatments. This result suggests that above a certain thr

SUBMITTER: Gouesbet G 

PROVIDER: S-EPMC123771 | biostudies-literature | 2002 Mar

REPOSITORIES: biostudies-literature