Triple-mode point-of-care diagnostics for high-risk human papillomavirus in urine.

ABSTRACT: Human papillomavirus (HPV) screening is crucial for early diagnosis and prevention of cervical cancer, yet fast and convenient HPV detection remains challenging, especially in resource-limited areas. Herein, we developed a nucleic acid test named SSMG-LAMP, which combined loop-mediated isothermal amplification (LAMP) with an engineered DNA indicator (SYBR Safe - Malachite Green) for the point-of-care diagnosis of high-risk HPV strains (HPV 16 and 18) in urine. The assay can be completed within 45 min, including DNA extraction, SSMG-LAMP reaction, and signal readout using a simple, portable system. This system enabled the triple-mode detection of DNA targets using colorimetry, fluorometry, and electrochemistry, and can detect as low as 10 copies μL-1 HPV DNA. As a preliminary validation, we

SUBMITTER: Thai DA 

PROVIDER: S-EPMC12377189 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature