Targeted deletions of large syntenic regions in <i>Arabidopsis thaliana</i>.

ABSTRACT: Plant genomes have undergone multiple rounds of whole-genome duplication (WGD) throughout their evolutionary history. As a result, many species, including Arabidopsis thaliana, retain duplicated genomic segments, or syntenic regions, which harbor large numbers of paralogous genes preserved from these ancient WGD events. We deleted four large, duplicated blocks, ranging from ~115 kb to ~684 kb using Staphylococcus aureus Cas9 to explore the effects of knocking out these blocks in Arabidopsis. Large deletions like these remain rare, especially in small and gene-dense plant genomes. Deletions were subsequently verified using whole-genome sequencing, which revealed minimal off-target effects. The number of deleted genes ranged from 16 to 60, and transposable elements range

SUBMITTER: Papikian A 

PROVIDER: S-EPMC12377758 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature