Characterization of recombinase activity across cellular growth phases.

ABSTRACT: Recombinases are essential enzymes in synthetic biology and biomedical research, enabling site-specific DNA modifications for applications such as gene therapy, the generation of transgenic models, and the construction of genetic circuits. Recombination efficiency depends on several factors, including intracellular recombinase concentration and the growth phase of the host cells. Although recombination is typically studied during exponential growth, the effects of stationary-phase dynamics on recombinase activity remain poorly understood. In this study, we examine how bacterial growth phase influences recombinase-mediated DNA modifications, using the serine recombinase Bxb1 as a model. We engineered a genetic system in Escherichia coli to quantify intracellular Bxb1 levels and to measure r

SUBMITTER: Gonzalez-Colell M 

PROVIDER: S-EPMC12378985 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature