A Dual-Readout Photonic Sensor for Simultaneous Measurement of Enzyme Activity and Concentration.

ABSTRACT: Enzyme assays are a cornerstone of basic biology and clinical diagnosis. Typically, enzyme activity is measured, but concentration of the enzyme is also of interest, as are comparisons between concentration and activity. In these situations, separate concentration (i.e., ELISA) and activity (i.e., absorbance) assays are required to fully quantify. Here, we report a multiplex disposable photonic biosensor for simultaneous measurement of enzyme activity and concentration. Capture of the enzyme by a ring resonator-bound antibody produces a red shift in resonance, which can be referenced to a nonspecific binding control. At the same time, enzyme-mediated degradation of a ring-bound substrate produces a resonance blue shift, which can be referenced to a peptide inert to enzymatic cleavage. We t

SUBMITTER: Butt JN 

PROVIDER: S-EPMC12379170 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature